AUI

AUI may stand for:


 * Ethernet's Attachment Unit Interface, a 15-pin D-connector
 * The constructed language aUI (language)
 * The National Rail code for Ardlui railway station, United Kingdom
 * Associated Universities, Inc., the corporation that operates the National Radio Astronomy Observatory (NRAO)
 * Amiga User International, a monthly magazine dedicated to the Amiga computer
 * Al Akhawayn University, a university located in Ifrane, Morocco
 * Adaptive user interface
 * Audible user interface, for blind people to use digital devices
 * Attentive user interface